Değişik patlıcan genotiplerinde in vitro androgenesis ve haploid bitki oluşumunu uyarıcı bazı etmenler üzerinde araştırmalar
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1991
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Sosyal Bilimler Enstitüsü
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On the obtention of haploid plants by anther culture in eggplant was studied. Effects of sugar, active charcoal and plant growth regulators as well as cold shock traitments before culture and high temperature shock treatments after culture and the effects of growing conditions of donor plants were investigated. In cytological investigation, the most availableIV development period for anther culture was found as the just previous period of 1st polen mitosis. The petal levels of the buds in this period was found as the level of sepals and the color of anther was observed as greenish yellow. A relationship between androgenesis and genotype in eggplants haploid plants could be obtained from Halep Karası and Baluroi Fi experimental cultivars only embrioids occured in Prelane Fi and Kemer cultivars, but not plants. No haploid embryos or plants could be obtained from the other cultivars (Dourga, Pala, Adana Topağı, Şeytan, Black Beauty, Marfa Fi, Fabina Fi, Galine Fi). The establishment medium prepared as 12 % saccarose, 5 mg/1 Kinetin and 5 mg/1 2,4-D according,to Chambonnet (1985) gave the best results. There wasn't a positive effect of active charcoal and cold applications to the buds before the cultivation, the best result was obtained from 8 days darkness after the plantation and +35°C temperature applied anthefrs. The propogation of the donor plants under short day and low temperature conditions didn't have a stimulative effect on androgenesis.The haploid plants were obtained from anthers of the young plants which were grown in spring or summer time. In different stages of the research 22 embryos and 13 haploid plants are obtained. Key Words: Eggplant, Solanum melongena L., anther culture, androgenesis, hormons, sugar, active charcoal, genotype, termique shocks, donor plant.
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